goat anti-protamine 2 (prm2)  (Santa Cruz Biotechnology)

Journal: Communications Biology

Article Title: Lysine malonylation regulates human sperm motility

doi: 10.1038/s42003-026-09683-y

Figure Lengend Snippet: A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: PRM2 for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.

Article Snippet: Anti-ACTIN antibody (66009-1-Ig), anti-SIRT5 antibody (67257-1-Ig), anti-acetyl-CoA carboxylase 1 (ACC1) antibody (21923-1-AP), anti-fatty acid synthase (FASN) antibody (10624-2-AP), anti-protamine 2 (PRM2) antibody (14500-1-AP), anti-GAPDHS (83290-3-RR) and anti-VDAC3 (82666-14-RR) were obtained from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Isolation, Western Blot, Staining, Immunofluorescence, Confocal Microscopy, Microscopy

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Integrative Proteomic and Phosphoproteomic Profiling of Testis from Wip1 Phosphatase-Knockout Mice: Insights into Mechanisms of Reduced Fertility* *

doi: 10.1074/mcp.RA117.000479

Figure Lengend Snippet: Wip1 deficiency disrupts spermatids development in the testis and reduces sperm count in the epididymis in mice. A, Overall testis sizes in 10-week-old Wip1-KO and WT mice. B, Weights of testes harvested from adult mice were reduced in Wip1-KO mice. Histological analyses of testes from 10-week-old Wip1-KO and WT mice at (C, a-b) low magnification (×10) and at (C, c-d) high magnification (×20). Immunohistochemical images of testis sections from 10-week-old WT and Wip1-KO mice stained with (C, e-f) anti-Prm2 antibody and (C, g-h) anti-MVH antibody. Hematoxylin-eosin (H&E)-stained sections of caput epididymides from 10-week-old WT and Wip1-KO mouse testes at (D, i-j) low magnification (×10) and at (D, k-l) high magnification (×20). (D, m-n) Transmission electron micrographs of caput epididymides from 10-week-old WT and Wip1-KO mice. (D, o-p) H&E-stained sections of cauda epididymides from 10-week-old WT and Wip1-KO mice. Data are expressed as mean ± S.E. **p < 0.01. Scale bars: 50 μm (Ca-d, Cg-h, Di-l, Do-p); 20 μm (Ce-f, Dm-n).

Article Snippet: For immunostaining, deparaffinized and rehydrated slides were incubated with boiling 0.1 m sodium citrate (pH 6.0) for 10 min for antigen retrieval, and stained with respective antibodies: rabbit anti-mouse VASA homologue (MVH) (Abcam, Cambridge, UK), goat anti-protamine 2 (Prm2) (Santa Cruz Biotechnology, Danvers, MA), rabbit anti-Wilms tumor (WT1) (Abcam), rabbit anti-β-catenin (Abcam), rabbit anti-occludin (Abcam), rabbit anti-N-cadherin (Abcam), and rabbit anti-zona occludens-1 (ZO-1) (Cell Signaling Technology).

Techniques: Immunohistochemical staining, Staining, Transmission Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients

doi: 10.1016/j.omtn.2017.09.007

Figure Lengend Snippet: Morphological and Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids (A–C) Phase-contrast microscope revealed the morphology of the freshly isolated human pachytene spermatocytes (A), spermatogonia (B), and round spermatids (C) of OA patients. (D–F) DIC microscope showed the morphological characteristics of the freshly isolated human pachytene spermatocytes (D), spermatogonia (E), and round spermatids (F) of OA patients. Scale bars, 20 μm (A–C) and 5 μm (D–F). (G) RT-PCR revealed the transcripts of GPR125 , RET , GFRA1 , THY1 , UCHL1 , MAGEA4 , and PLZF in the fleshly isolated spermatogonia, the expression of SYCP3 and SYCP1 in pachytene spermatocytes, and mRNA of TNP1 , TNP2 , PRM1 , PRM2 , and ACR in round spermatids. RNA without RT (RT-) but with PCR of GAPDH primers was utilized as negative controls, and GAPDH served as loading controls of total RNA.

Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and Protamine 2 (PRM2) (Atlas Antibodies, HPA056386, 1:200).

Techniques: Isolation, Microscopy, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Molecular Therapy. Nucleic Acids

Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients

doi: 10.1016/j.omtn.2017.09.007

Figure Lengend Snippet: Phenotypic Characterization of Freshly Isolated Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids (A–D) Immunocytochemistry revealed the expression of THY1 (A), GFRA1 (B), PLZF (C), and UCHL1 (D) in the freshly isolated human spermatogonia. Scale bars, 10 μm. (E) Meiotic chromatin spread by triple immunostaining displayed the co-expression of CREST, SYCP3, and MLH1 in the freshly isolated human pachytene spermatocytes. Scale bar, 5 μm. (F–I) Immunocytochemistry demonstrated the expression of PNA (F), PRM2 (G), and rabbit IgG (I) in the freshly isolated human round spermatids and rabbit IgG (H) in the freshly isolated human spermatogonia. Scale bars, 10 μm.

Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and Protamine 2 (PRM2) (Atlas Antibodies, HPA056386, 1:200).

Techniques: Isolation, Immunocytochemistry, Expressing, Triple Immunostaining

Journal: Molecular Therapy. Nucleic Acids

Article Title: Distinct Expression Profiles and Novel Targets of MicroRNAs in Human Spermatogonia, Pachytene Spermatocytes, and Round Spermatids between OA Patients and NOA Patients

doi: 10.1016/j.omtn.2017.09.007

Figure Lengend Snippet: The Sequences of Gene Primers Used for RT-PCR and Real-Time PCR

Article Snippet: The primary antibodies included THY1 (Abcam, ab133350, 1:200), GFRA1 (Santa Cruz, sc-6156, 1:200), PLZF (Santa Cruz, sc-22839, 1:200), UCHL1 (Bio-Rad, MCA4750, 1:200), PNA (Life Technologies, L32458, 1:200), and Protamine 2 (PRM2) (Atlas Antibodies, HPA056386, 1:200).

Techniques: